This lesson is still being designed and assembled (Pre-Alpha version)

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Introduction to Workflows with Common Workflow Language

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Debugging Workflows

Overview

Teaching: 0 min
Exercises: 0 min
Questions

  • introduce within above lessons?

Objectives

  • interpret commonly encountered error messages

  • solve these common issues

Learning objectives

By the end of this episode, learners should be able to recognize and fix simple bugs in their workflow code.

When working on a CWL workflow, you will probably encounter errors. There are many different errors possible. It is always very important to check the error message in the terminal, because it will give you information on the error. This error message will give you the type of error as well as the line of code that contains the error. Some of these errors will be explained in this episode.

As a first step to check if your CWL script contains any errors, you can run the workflow with the --validate flag.

cwltool --validate CWL_SCRIPT.cwl

It is possible that the script is validated, however, it still gets an error. If you encounter an error, the best practice is to run the workflow with the --debug flag. This will provide you with extensive information on the error you encounter.

cwltool --debug CWL_SCRIPT.cwl

YAML errors

First of all, errors in the YAML syntax. When writing a piece of code, it is very easy to make a mistake.

Some very common YAML errors are:

Wiring error

Wiring errors often occur when you forget to add an output from a workflow’s step to the outputs section. This doesn’t cause an error message, but there won’t be any output in your directory. To get the desired output you have to run the workflow again. Best practice is to check your outputs section before running your script to make sure all the outputs you want are there.

Type mismatch

Type errors take place when there is a mismatch in type between variables. When you declare a variable in the inputs section, the type of this variable has to match the type in the YAML inputs file and the type used in one of the workflows steps. The error message that is shown when this error occurs will tell you on which line the mismatch happens.

cwlVersion: v1.2
class: Workflow

inputs:
  rna_reads_human: int
  ref_genome: Directory
  annotations: File

steps:
  quality_control:
    run: bio-cwl-tools/fastqc/fastqc_2.cwl
    in:
      reads_file: rna_reads_human
    out: [html_file]

  mapping_reads:
    requirements:
      ResourceRequirement:
        ramMin: 9000
    run: bio-cwl-tools/STAR/STAR-Align.cwl
    in:
      RunThreadN: {default: 4}
      GenomeDir: ref_genome
      ForwardReads: rna_reads_human
      OutSAMtype: {default: BAM}
      SortedByCoordinate: {default: true}
      OutSAMunmapped: {default: Within}
    out: [alignment]

  index_alignment:
    run: bio-cwl-tools/samtools/samtools_index.cwl
    in:
      bam_sorted: mapping_reads/alignment
    out: [bam_sorted_indexed]

  count_reads:
    requirements:
      ResourceRequirement:
        ramMin: 500
    run: bio-cwl-tools/subread/featureCounts.cwl
    in:
      mapped_reads: index_alignment/bam_sorted_indexed
      annotations: annotations
    out: [featurecounts]

outputs:
  qc_html:
    type: File
    outputSource: quality_control/html_file
  bam_sorted_indexed:
    type: File
    outputSource: index_alignment/bam_sorted_indexed
  featurecounts:
    type: File
    outputSource: count_reads/featurecounts

$ cwltool rna_seq_workflow.cwl workflow_input.yml

ERROR Tool definition failed validation:

rna_seq_workflow.cwl:5:3:   Source 'rna_reads_human' of type "int" is incompatible
rna_seq_workflow.cwl:24:7:   with sink 'ForwardReads' of type ["File", {"type": "array", "items":
                             "File"}]
rna_seq_workflow.cwl:5:3:   Source 'rna_reads_human' of type "int" is incompatible
rna_seq_workflow.cwl:13:7:   with sink 'reads_file' of type ["File"]

Format error

Some files need a specific format that needs to be specified in the YAML inputs file, for example the fastq file in the RNA-seq analysis. When you don’t specify a format, an error will occur. You can for example use the EDAM ontology.

rna_reads_human:
  class: File
  location: rnaseq/raw_fastq/Mov10_oe_1.subset.fq
ref_genome:
  class: Directory
  location: rnaseq/hg19-chr1-STAR-index
annotations:
  class: File
  location: rnaseq/reference_data/chr1-hg19_genes.gtf

$ cwltool rna_seq_workflow.cwl workflow_input.yml

ERROR Exception on step 'mapping_reads'
ERROR [step mapping_reads] Cannot make job: Expected value of 'ForwardReads' to have format http://edamontology.org/format_1930 but
  File has no 'format' defined: {
    "class": "File",
    "location": "file:///home/mbexegc2/Documents/projects/bioexcel/follow-cwl-novice-tutorial/novice-tutorial-exercises/rnaseq/raw_fastq/Mov10_oe_1.subset.fq",
    "size": 75706556,
    "basename": "Mov10_oe_1.subset.fq",
    "nameroot": "Mov10_oe_1.subset",
    "nameext": ".fq"
}

Key Points

  • First key point. Brief Answer to questions. (FIXME)

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